Urokinase/Urokinase Alteplase/Urokinase/U-Plasminogen Activator/Urokinase

English Name: Urokinase from human urine

Other Names: Urokinase; Urokinase; U-plasminogen activator

CAS Number: 9039-53-6

Grade: BR

Specific Activity: ≥120,000 IU/mg

Potency: ≥75,000 IU/mg

Activity Definition: One unit is the amount of enzyme required to hydrolyze 1 μmole of Be-Ala-Gly-Arg-4Na per minute at 37°C, pH 8.8

Solubility: Completely dissolves within 60 seconds (3000 IU/ml)

High Molecular Weight Content: ≥85% (M.W. 54,000)

Pyrogen: Complies with specifications (20,000 IU/ml·kg)

Endotoxin: ≤2 EU/20,000 IU

Abnormal Toxicity: Negative (2500 IU/0.5 ml/mouse)

HBs-Ag: Negative (50,000 IU/ml)

Anti-HIV I + II: Negative (50,000 IU/ml)

Anti-HCV Abs: Negative (50,000 IU/ml)

Appearance: White or off-white lyophilized powder. A proteolytic enzyme derived from human urine or kidney cells. Its natural substrate is plasminogen. Sensitive to moisture. Soluble in water. The dry powder is stable at 4°C, while the aqueous solution is stable for 3 days at 4°C.

Use: Biochemical research. It is a protease that cleaves plasminogen to form plasmin. It binds to cell surface receptors (uPAR), regulating cell adhesion, tissue remodeling, and migration, while also activating intracellular signaling pathways. Urokinase consists of three domains: an N-terminal domain homologous to epidermal growth factor, a central kringle domain, and a C-terminal protease domain.

Storage: -20°C